TY - JOUR
KW - Cytoplasm
KW - Humans
KW - Interleukin-15
KW - leprosy
KW - Monocytes
KW - Mycobacterium leprae
KW - RNA, Messenger
KW - T-Lymphocytes
AU - Jullien D
AU - Sieling P A
AU - Uyemura K
AU - Mar N D
AU - Rea T H
AU - Modlin R L
AB - <p>IL-15 is a novel cytokine with potent T cell growth factor activity. Here, we investigated the role of IL-15 in the human immune response to intracellular infection by studying patients leprosy. We found that IL-15 mRNA and protein were more strongly expressed in immunologically resistant tuberculoid patients than in with unresponsive and susceptible lepromatous patients. In vitro, Mycobacterium leprae induced IL-15 secretion from peripheral blood monocytes. Furthermore, rIL-15 by itself and in combination with rIL-2 or rIL-7 augmented PBMC proliferative responses to the pathogen. Although rIL-15 expanded the CD3-CD56+ (NK) subset, rIL-15 combined with M. leprae induced the expansion of CD3+CD56+ T cells. Immunohistologic analysis of leprosy skin lesions indicated that the frequency of CD56+ cells was greatest in the group of patients with high IL-15 expression, and that >90% of the CD56+ cells in lesions were CD3+ T cells. Therefore, IL-15 augments the local T cell response to human intracellular pathogen.</p>
BT - Journal of immunology (Baltimore, Md. : 1950)
C1 - http://www.ncbi.nlm.nih.gov/pubmed/8992997?dopt=Abstract
DA - 1997 Jan 15
IS - 2
J2 - J. Immunol.
LA - eng
N2 - <p>IL-15 is a novel cytokine with potent T cell growth factor activity. Here, we investigated the role of IL-15 in the human immune response to intracellular infection by studying patients leprosy. We found that IL-15 mRNA and protein were more strongly expressed in immunologically resistant tuberculoid patients than in with unresponsive and susceptible lepromatous patients. In vitro, Mycobacterium leprae induced IL-15 secretion from peripheral blood monocytes. Furthermore, rIL-15 by itself and in combination with rIL-2 or rIL-7 augmented PBMC proliferative responses to the pathogen. Although rIL-15 expanded the CD3-CD56+ (NK) subset, rIL-15 combined with M. leprae induced the expansion of CD3+CD56+ T cells. Immunohistologic analysis of leprosy skin lesions indicated that the frequency of CD56+ cells was greatest in the group of patients with high IL-15 expression, and that >90% of the CD56+ cells in lesions were CD3+ T cells. Therefore, IL-15 augments the local T cell response to human intracellular pathogen.</p>
PY - 1997
SP - 800
EP - 6
T2 - Journal of immunology (Baltimore, Md. : 1950)
TI - IL-15, an immunomodulator of T cell responses in intracellular infection.
VL - 158
SN - 0022-1767
ER -