TY - JOUR
KW - Case-Control Studies
KW - Demyelinating Diseases
KW - Glycoproteins
KW - Humans
KW - leprosy
KW - Metals
KW - Molecular Weight
KW - Nerve Tissue Proteins
KW - Peripheral nerves
KW - Phosphorylation
KW - Reference Values
AU - Suneetha L M
AU - Korula R J
AU - Balasubramanian A S
AB - <p>Protein phosphorylation in a low speed supernatant of human peripheral nerve (tibial and sural) homogenate was investigated. The major phosphorylated proteins had molecular mass in the range of 70, 55, 45, and 25 kDa. Mg2+ or Mn2+ was essential for maximum phosphorylation although Zn2+, Co2+, and Ca2+ could partially support phosphorylation. External protein substrates casein and histone were also phosphorylated. The protein phosphatase inhibitor orthovanadate enhanced the phosphorylation of the 45 and 25 kDa proteins significantly. Concanavalin A-Sepharose chromatography of the phosphorylated peripheral nerve proteins showed that the 25 kDa protein was a glycoprotein. Protein phosphorylation of peripheral nerves from leprosy affected individuals was compared with normals. The phosphorylation of 25 kDa protein was decreased in most of the patients with leprosy.</p>
BT - Neurochemical research
C1 - http://www.ncbi.nlm.nih.gov/pubmed/8829144?dopt=Abstract
DA - 1996 Jun
DO - 10.1007/bf02527729
IS - 6
J2 - Neurochem. Res.
LA - eng
N2 - <p>Protein phosphorylation in a low speed supernatant of human peripheral nerve (tibial and sural) homogenate was investigated. The major phosphorylated proteins had molecular mass in the range of 70, 55, 45, and 25 kDa. Mg2+ or Mn2+ was essential for maximum phosphorylation although Zn2+, Co2+, and Ca2+ could partially support phosphorylation. External protein substrates casein and histone were also phosphorylated. The protein phosphatase inhibitor orthovanadate enhanced the phosphorylation of the 45 and 25 kDa proteins significantly. Concanavalin A-Sepharose chromatography of the phosphorylated peripheral nerve proteins showed that the 25 kDa protein was a glycoprotein. Protein phosphorylation of peripheral nerves from leprosy affected individuals was compared with normals. The phosphorylation of 25 kDa protein was decreased in most of the patients with leprosy.</p>
PY - 1996
SP - 707
EP - 12
T2 - Neurochemical research
TI - Protein phosphorylation in human peripheral nerve: altered phosphorylation of a 25-kDa glycoprotein in leprosy.
VL - 21
SN - 0364-3190
ER -