TY - JOUR KW - Antibodies KW - Cells, Cultured KW - Dinoprostone KW - Humans KW - Immune Tolerance KW - Indomethacin KW - Interleukin-10 KW - Leprosy, lepromatous KW - Lymphocyte Activation KW - Lymphopenia KW - Monocytes KW - Th1 Cells AU - Misra N AU - Selvakumar M AU - Singh S AU - Bharadwaj M AU - Ramesh V AU - Misra R S AU - Nath I AB -

Our previous studies had shown that the clinicopathological spectrum in leprosy was associated with discrete T cell subsets in circulation, with tuberculoid patients having antigen-induced Th 1, whereas lepromatous leprosy patients with antigen-specific T cell anergy possessed Th 2 cells. The present study shows that infected monocytes from lepromatous but not tuberculoid leprosy patients released soluble factors (MoF(s)) containing IL-10 and PGE2 which inhibited M. leprae induced in vitro lymphoproliferation of previously sensitised healthy or tuberculoid leprosy subjects. A strong negative correlation was observed between adherent cell derived IL-10 and IL-2 at the level of both the product and cytokine mRNA. Moreover, anti-IL-10 antibodies and indomethacin partially reversed the suppressor effects of MoF(s). Taken together these studies indicate that infected monocytes contribute to the development of T cell anergy by releasing factors that affect regulatory cytokines and T cell subset differentiation in lepromatous leprosy.

BT - Immunology letters C1 - http://www.ncbi.nlm.nih.gov/pubmed/8719110?dopt=Abstract CN - MISRA 1995 DA - 1995 Dec DO - 10.1016/0165-2478(95)02455-7 IS - 2 J2 - Immunol. Lett. LA - eng N2 -

Our previous studies had shown that the clinicopathological spectrum in leprosy was associated with discrete T cell subsets in circulation, with tuberculoid patients having antigen-induced Th 1, whereas lepromatous leprosy patients with antigen-specific T cell anergy possessed Th 2 cells. The present study shows that infected monocytes from lepromatous but not tuberculoid leprosy patients released soluble factors (MoF(s)) containing IL-10 and PGE2 which inhibited M. leprae induced in vitro lymphoproliferation of previously sensitised healthy or tuberculoid leprosy subjects. A strong negative correlation was observed between adherent cell derived IL-10 and IL-2 at the level of both the product and cytokine mRNA. Moreover, anti-IL-10 antibodies and indomethacin partially reversed the suppressor effects of MoF(s). Taken together these studies indicate that infected monocytes contribute to the development of T cell anergy by releasing factors that affect regulatory cytokines and T cell subset differentiation in lepromatous leprosy.

PY - 1995 SP - 123 EP - 28 T2 - Immunology letters TI - Monocyte derived IL 10 and PGE2 are associated with the absence of Th 1 cells and in vitro T cell suppression in lepromatous leprosy. VL - 48 SN - 0165-2478 ER -