TY - JOUR
KW - Animals
KW - Dapsone
KW - Humans
KW - Immunoenzyme Techniques
KW - Leprostatic Agents
KW - leprosy
KW - Macrophages
KW - Mice
KW - Nerve Tissue
KW - Rabbits
KW - Rifampin
KW - Schwann Cells
KW - Skin
AU - Save M P
AU - Shetty V P
AU - Antia N H
AB - <p>Intracellular localization of antileprosy drugs dapsone (DDS) and rifampicin (RFP) was carried out on skin and nerve lesions obtained from multidrug treated, multi (BL-LL)- and pauci (BT-TT) bacillary cases of leprosy using immunocytochemical techniques. Intracellular localization of the above drugs especially in macrophages and Schwann cells was aimed by using rabbit raised anti DDS and RFP polyclonal antibody in an indirect peroxidase assay. Our study records both intra and extracellular staining with anti DDS and RFP antibodies in the skin as well as nerve lesions of MB and PB cases treated with MDT. All the nerves under investigation had moderate to severe pathology and hence free diffusion of the drug could be attributed to the broken barrier. Basal lamina around the Schwann cell did not seem to form a barrier. It was also noted that the drug (metabolite) persisted over a long period of time).</p>
BT - Indian journal of leprosy
C1 - http://www.ncbi.nlm.nih.gov/pubmed/8576606?dopt=Abstract
CN - Infolep Library - available
DA - 1995 Jul-Sep
IS - 3
J2 - Indian J Lepr
LA - eng
N2 - <p>Intracellular localization of antileprosy drugs dapsone (DDS) and rifampicin (RFP) was carried out on skin and nerve lesions obtained from multidrug treated, multi (BL-LL)- and pauci (BT-TT) bacillary cases of leprosy using immunocytochemical techniques. Intracellular localization of the above drugs especially in macrophages and Schwann cells was aimed by using rabbit raised anti DDS and RFP polyclonal antibody in an indirect peroxidase assay. Our study records both intra and extracellular staining with anti DDS and RFP antibodies in the skin as well as nerve lesions of MB and PB cases treated with MDT. All the nerves under investigation had moderate to severe pathology and hence free diffusion of the drug could be attributed to the broken barrier. Basal lamina around the Schwann cell did not seem to form a barrier. It was also noted that the drug (metabolite) persisted over a long period of time).</p>
PY - 1995
SP - 273
EP - 84
T2 - Indian journal of leprosy
TI - Intracellular localization of dapsone and rifampicin in skin and nerve of multidrug treated leprosy patients.
VL - 67
SN - 0254-9395
ER -