TY - JOUR KW - Adult KW - Base Sequence KW - DNA, Bacterial KW - Female KW - Humans KW - Male KW - Middle Aged KW - Molecular Sequence Data KW - Mycobacterium leprae KW - Peripheral nerves KW - polymerase chain reaction AU - Chemouilli P AU - Woods S AU - Said G AU - Cole S T AB -

A simple procedure is described for the detection of Mycobacterium leprae by the polymerase chain reaction in nerve biopsies sectioned with a cryostat and then treated with proteinase K. All samples from lepromatous leprosy patients and the majority of samples from paucibacillary cases yielded positive results. This approach may be useful for differentiating between leprosy and other inflammatory neuropathies.

BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 -

http://www.ncbi.nlm.nih.gov/pubmed/8627108?dopt=Abstract

DA - 1996 Mar IS - 1 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -

A simple procedure is described for the detection of Mycobacterium leprae by the polymerase chain reaction in nerve biopsies sectioned with a cryostat and then treated with proteinase K. All samples from lepromatous leprosy patients and the majority of samples from paucibacillary cases yielded positive results. This approach may be useful for differentiating between leprosy and other inflammatory neuropathies.

PY - 1996 SP - 1 EP - 5 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - Detection of Mycobacterium leprae in nerve lesions by the polymerase chain reaction. UR - http://ila.ilsl.br/pdfs/v64n1a01.pdf VL - 64 SN - 0148-916X ER -