TY - JOUR
KW - Biopsy
KW - Colorimetry
KW - DNA, Bacterial
KW - Electrophoresis, Agar Gel
KW - Humans
KW - leprosy
KW - Mycobacterium leprae
KW - polymerase chain reaction
KW - Reproducibility of Results
KW - Sensitivity and Specificity
KW - Skin
AU - Vliet G M
AU - Wit M Y
AU - Klatser P R
AB - <p>A colorimetric assay for the detection of PCR-products is described. The assay is based on amplification of DNA in the presence of digoxigenin-dUTP. After immobilization of the PCR products to a microtitre plate, amplified DNA could be detected colorimetrically. The sensitivity of this colorimetric assay was equal to gel-analysis allowing the detection of 100 fg of template DNA. Here, we show that it can be used to detect Mycobacterium leprae DNA in biopsy specimens from leprosy patients. The simplicity and the low degree of variation make this assay an alternative to gel-analysis.</p>
BT - Molecular and cellular probes
C1 - http://www.ncbi.nlm.nih.gov/pubmed/8455642?dopt=Abstract
DA - 1993 Feb
DO - 10.1006/mcpr.1993.1008
IS - 1
J2 - Mol. Cell. Probes
LA - eng
N2 - <p>A colorimetric assay for the detection of PCR-products is described. The assay is based on amplification of DNA in the presence of digoxigenin-dUTP. After immobilization of the PCR products to a microtitre plate, amplified DNA could be detected colorimetrically. The sensitivity of this colorimetric assay was equal to gel-analysis allowing the detection of 100 fg of template DNA. Here, we show that it can be used to detect Mycobacterium leprae DNA in biopsy specimens from leprosy patients. The simplicity and the low degree of variation make this assay an alternative to gel-analysis.</p>
PY - 1993
SP - 61
EP - 6
T2 - Molecular and cellular probes
TI - A simple colorimetric assay for detection of amplified Mycobacterium leprae DNA.
VL - 7
SN - 0890-8508
ER -