TY - JOUR KW - Computers KW - DNA KW - Fibroblasts KW - Histocytochemistry KW - Humans KW - leprosy KW - Lymphocytes KW - Microscopy, Electron, Scanning KW - Photometry KW - Staining and Labeling AU - Vishnevetskiĭ F E AB -

DNA of leprosy cells in serial paraffine sections stained after Felgen were studied photometrically in scanning microscope SPM with electron computer Wang 720C. Significant differences in extinction values (up to 30--35%) between individual sections within the same series were revealed. Cumulative results of different series were rather uniform. It was concluded that photometric studies of serial sections should be carried out for reliable determination of DNA.

BT - Biulleten' eksperimental'noi biologii i meditsiny C1 - http://www.ncbi.nlm.nih.gov/pubmed/83169?dopt=Abstract DA - 1978 Dec IS - 12 J2 - Biull Eksp Biol Med LA - rus N2 -

DNA of leprosy cells in serial paraffine sections stained after Felgen were studied photometrically in scanning microscope SPM with electron computer Wang 720C. Significant differences in extinction values (up to 30--35%) between individual sections within the same series were revealed. Cumulative results of different series were rather uniform. It was concluded that photometric studies of serial sections should be carried out for reliable determination of DNA.

PY - 1978 SP - 742 EP - 4 T2 - Biulleten' eksperimental'noi biologii i meditsiny TI - [Cytophotometry of DNA in serial sections]. VL - 86 SN - 0365-9615 ER -