TY - JOUR KW - Adolescent KW - Adult KW - Aged KW - Aged, 80 and over KW - Antibodies, Bacterial KW - Antigens, Bacterial KW - Bacterial Proteins KW - Child KW - Child, Preschool KW - Cross Reactions KW - Enzyme-Linked Immunosorbent Assay KW - Glycolipids KW - Humans KW - leprosy KW - Middle Aged KW - Mycobacterium bovis KW - Mycobacterium leprae KW - Sensitivity and Specificity KW - Tuberculosis AU - Vikerfors T AU - Olcén P AU - Wiker H AU - Watson J D AB -

Two enzyme-linked immunosorbent assays (ELISAs) for the detection of leprosy antibodies were developed. These assays were based on the recombinant 18-kDa protein of Mycobacterium leprae and the 85B antigen of M. bovis. Sera from leprosy and tuberculosis patients were analyzed, and a phenolic glycolipid-I (PGL-I) ELISA was used as a reference test. The 18-kDa ELISA reached a sensitivity of 70% on lepromatous leprosy sera. The corresponding results for the 85B and the PGL-I ELISAs were 93% and 96%, respectively. The sensitivity on tuberculoid leprosy sera was 72% for the 18-kDa antigen, 95% for the 85B antigen, and 60% for the PGL-I antigen. The 18-kDa antigen ELISA was found to have crossreactivity to sera from patients with tuberculosis, while the 85B and PGL-I assays had high specificity.

BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 -

http://www.ncbi.nlm.nih.gov/pubmed/8151188?dopt=Abstract

DA - 1993 Dec IS - 4 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -

Two enzyme-linked immunosorbent assays (ELISAs) for the detection of leprosy antibodies were developed. These assays were based on the recombinant 18-kDa protein of Mycobacterium leprae and the 85B antigen of M. bovis. Sera from leprosy and tuberculosis patients were analyzed, and a phenolic glycolipid-I (PGL-I) ELISA was used as a reference test. The 18-kDa ELISA reached a sensitivity of 70% on lepromatous leprosy sera. The corresponding results for the 85B and the PGL-I ELISAs were 93% and 96%, respectively. The sensitivity on tuberculoid leprosy sera was 72% for the 18-kDa antigen, 95% for the 85B antigen, and 60% for the PGL-I antigen. The 18-kDa antigen ELISA was found to have crossreactivity to sera from patients with tuberculosis, while the 85B and PGL-I assays had high specificity.

PY - 1993 SP - 571 EP - 80 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - Serological response in leprosy and tuberculosis patients to the 18-kDa antigen of Mycobacterium leprae and antigen 85B of Mycobacterium bovis BCG. UR - http://ila.ilsl.br/pdfs/v61n4a06.pdf VL - 61 SN - 0148-916X ER -