TY - JOUR KW - Animals KW - Antigens, Bacterial KW - BCG Vaccine KW - Cell Fractionation KW - Cross Reactions KW - Cytoplasm KW - Diagnosis, Differential KW - Guinea Pigs KW - Humans KW - leprosy KW - Lipase KW - Mycobacterium bovis KW - Mycobacterium leprae KW - Pronase KW - Skin Tests KW - Trypsin AU - Elliston E P AU - TAYLOR C E AB -

A successful method for purification of M. leprae from human leproma without subjection to heat has been developed. The "floater" phenomenon has been described which consists of bacillary tendency to float in the supernate when bacilli which are not autoclaved are separated from tissues by enzymatic digestion. A method for preparing cytoplasmic fractions from purified M. leprae has been developed for the production of a skin test antigen for leprosy. The cytoplasmic fraction of M. leprae elicited positive skin test responses in people with tuberculoid leprosy and negative responses in lepromatous leprosy. Cytoplasmic preparations from purified M. leprae had little cross-reactive relationship with the organism BCG. The small particulate fraction elicited positive reactions in PPD-S negative as well as BCG vaccinated individuals.

BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 -

http://www.ncbi.nlm.nih.gov/pubmed/789262?dopt=Abstract

DA - 1976 Jul-Sep IS - 3 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -

A successful method for purification of M. leprae from human leproma without subjection to heat has been developed. The "floater" phenomenon has been described which consists of bacillary tendency to float in the supernate when bacilli which are not autoclaved are separated from tissues by enzymatic digestion. A method for preparing cytoplasmic fractions from purified M. leprae has been developed for the production of a skin test antigen for leprosy. The cytoplasmic fraction of M. leprae elicited positive skin test responses in people with tuberculoid leprosy and negative responses in lepromatous leprosy. Cytoplasmic preparations from purified M. leprae had little cross-reactive relationship with the organism BCG. The small particulate fraction elicited positive reactions in PPD-S negative as well as BCG vaccinated individuals.

PY - 1976 SP - 319 EP - 31 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - Separation of M. leprae from human leproma and the development of a cytoplasmic skin test antigen from purified bacilli. UR - http://ila.ilsl.br/pdfs/v44n3a05.pdf VL - 44 SN - 0148-916X ER -