TY - JOUR KW - Adolescent KW - Adult KW - Aged KW - Antigens, Bacterial KW - BCG Vaccine KW - Bacterial Proteins KW - Female KW - Humans KW - leprosy KW - Male KW - Middle Aged KW - Mycobacterium tuberculosis KW - T-Lymphocytes KW - Tuberculosis KW - Vaccination AU - Roche P W AU - Triccas J A AU - Avery D T AU - Fifis T AU - Billman-Jacobe H AU - Britton W J AB -

The immune responses of healthy recipients of Mycobacterium bovis bacille Calmette-Guérin (BCG) vaccine, tuberculosis (TB) patients, and contacts of TB patients were examined to three major secretory proteins of Mycobacterium tuberculosis, MPB59, MPB64, and MPB70. MPB59 evoked a T cell response in 78% of BCG vaccines, 62% of TB patients, and 60% of contacts. MPB64 and MPB70 were recognized by < 15% of BCG vaccinees, half of TB patients, and three-quarters of contacts. TB and leprosy patients had antibody responses to MPB59, but few had antibodies to MPB64 or MPB70. Hybridization of mycobacterial DNA with specific gene probes demonstrated the absence of a gene for MBP64 in the vaccine strain of BCG, but the MPB70 gene was found in all virulent and vaccine BCG strains tested. Since MPB64 and MPB70 can induce delayed-type hypersensitivity reactions in infected animals, either of these proteins may have potential as skin test reagents for detecting infection with M. tuberculosis.

BT - The Journal of infectious diseases C1 - http://www.ncbi.nlm.nih.gov/pubmed/7963739?dopt=Abstract DA - 1994 Nov DO - 10.1093/infdis/170.5.1326 IS - 5 J2 - J. Infect. Dis. LA - eng N2 -

The immune responses of healthy recipients of Mycobacterium bovis bacille Calmette-Guérin (BCG) vaccine, tuberculosis (TB) patients, and contacts of TB patients were examined to three major secretory proteins of Mycobacterium tuberculosis, MPB59, MPB64, and MPB70. MPB59 evoked a T cell response in 78% of BCG vaccines, 62% of TB patients, and 60% of contacts. MPB64 and MPB70 were recognized by < 15% of BCG vaccinees, half of TB patients, and three-quarters of contacts. TB and leprosy patients had antibody responses to MPB59, but few had antibodies to MPB64 or MPB70. Hybridization of mycobacterial DNA with specific gene probes demonstrated the absence of a gene for MBP64 in the vaccine strain of BCG, but the MPB70 gene was found in all virulent and vaccine BCG strains tested. Since MPB64 and MPB70 can induce delayed-type hypersensitivity reactions in infected animals, either of these proteins may have potential as skin test reagents for detecting infection with M. tuberculosis.

PY - 1994 SP - 1326 EP - 30 T2 - The Journal of infectious diseases TI - Differential T cell responses to mycobacteria-secreted proteins distinguish vaccination with bacille Calmette-Guérin from infection with Mycobacterium tuberculosis. VL - 170 SN - 0022-1899 ER -