TY - JOUR
KW - Antigens, Bacterial
KW - Case-Control Studies
KW - Cross Reactions
KW - Humans
KW - leprosy
KW - Mycobacterium leprae
KW - Precipitin Tests
AU - Kode J A
AU - Chiplunkar S V
AU - Samson P D
AU - Deo M G
AU - Gangal S G
AB - <p>Pooled sera from leprosy patients across the clinical spectrum, tuberculosis patients and healthy individuals were tested for their reactivity with antigens of Mycobacterium leprae and a panel of cultivable mycobacteria by immunoprecipitation technique. Sera from lepromatous leprosy patients demonstrated exclusive reactivity with the 26-kDa protein of M. tuberculosis H37Ra, 28-kDa protein of M. kansasii, 45-kDa protein of M. smegmatis, and 158, 40 and 14 kDa proteins of M. phlei. Sera from patients with borderline tuberculoid leprosy, tuberculoid leprosy, tuberculosis and health individuals failed to identify these antigens. Our studies indicate that analysis and characterization of immunodominant antigenic epitopes present on proteins of cultivable mycobacteria, sharing cross-reactive epitopes with M. leprae may prove to be important in the serodiagnosis of multibacillary leprosy as well as for developing vaccines for immunotherapy of leprosy.</p>
BT - Acta leprologica
C1 - http://www.ncbi.nlm.nih.gov/pubmed/7863757?dopt=Abstract
DA - 1994
IS - 2
J2 - Acta Leprol
LA - eng
N2 - <p>Pooled sera from leprosy patients across the clinical spectrum, tuberculosis patients and healthy individuals were tested for their reactivity with antigens of Mycobacterium leprae and a panel of cultivable mycobacteria by immunoprecipitation technique. Sera from lepromatous leprosy patients demonstrated exclusive reactivity with the 26-kDa protein of M. tuberculosis H37Ra, 28-kDa protein of M. kansasii, 45-kDa protein of M. smegmatis, and 158, 40 and 14 kDa proteins of M. phlei. Sera from patients with borderline tuberculoid leprosy, tuberculoid leprosy, tuberculosis and health individuals failed to identify these antigens. Our studies indicate that analysis and characterization of immunodominant antigenic epitopes present on proteins of cultivable mycobacteria, sharing cross-reactive epitopes with M. leprae may prove to be important in the serodiagnosis of multibacillary leprosy as well as for developing vaccines for immunotherapy of leprosy.</p>
PY - 1994
SP - 89
EP - 94
T2 - Acta leprologica
TI - Immunoprecipitation of mycobacterial antigens with sera from patients with leprosy.
VL - 9
SN - 0001-5938
ER -