For elimination of contaminants in a homogenate of nude-mouse footpad infected with M.leprae, the pretreatment with trypsin was tested. The results obtained indicated that the final sterile product was satisfactorily obtained, if the pretreatment with trypsin 0.05%, 37 degrees C, 60min to the homogenate was carried out before the treatment with sodium hydroxide 1%, 37 degrees C, 15min. This procedure might contribute to make a suspension of partially purified M.leprae from the infected tissues, because both the treatments with trypsin and sodium hydroxide could eliminate the infected tissues surrounding M.leprae as well as any contaminants, except other mycobacteria, present in a tissue homogenate.
BT - Nihon Rai Gakkai zasshi C1 - http://www.ncbi.nlm.nih.gov/pubmed/7844062?dopt=Abstract DA - 1994 Jul IS - 2 J2 - Nihon Rai Gakkai Zasshi LA - jpn N2 -For elimination of contaminants in a homogenate of nude-mouse footpad infected with M.leprae, the pretreatment with trypsin was tested. The results obtained indicated that the final sterile product was satisfactorily obtained, if the pretreatment with trypsin 0.05%, 37 degrees C, 60min to the homogenate was carried out before the treatment with sodium hydroxide 1%, 37 degrees C, 15min. This procedure might contribute to make a suspension of partially purified M.leprae from the infected tissues, because both the treatments with trypsin and sodium hydroxide could eliminate the infected tissues surrounding M.leprae as well as any contaminants, except other mycobacteria, present in a tissue homogenate.
PY - 1994 SP - 47 EP - 50 T2 - Nihon Rai Gakkai zasshi TI - [Elimination of contaminants in a homogenate of nude-mouse footpad experimentally infected with Mycobacterium leprae]. VL - 63 SN - 0386-3980 ER -