Immunohistochemistry reaction (Peroxidase anti-peroxidase--PAP) was carried out on fifty-two skin biopsies from leprosy patients with the purpose to identify the antigenic pattern in mycobacteria and to study the sensitivity of this method. Five different patterns were found: bacillar, granular, vesicular, cytoplasmatic and deposits, classified according to the antigenic material characteristics. Deposits (thinely particulate material) appeared more frequently, confirming the immunohistochemistry sensitivity to detect small amounts of antigens even when this material is not detected by histochemical stainings.
BT - Revista do Instituto de Medicina Tropical de Sao Paulo C1 - http://www.ncbi.nlm.nih.gov/pubmed/7569643?dopt=Abstract DA - 1995 Jan-Feb DO - 10.1590/s0036-46651995000100002 IS - 1 J2 - Rev. Inst. Med. Trop. Sao Paulo LA - eng N2 -Immunohistochemistry reaction (Peroxidase anti-peroxidase--PAP) was carried out on fifty-two skin biopsies from leprosy patients with the purpose to identify the antigenic pattern in mycobacteria and to study the sensitivity of this method. Five different patterns were found: bacillar, granular, vesicular, cytoplasmatic and deposits, classified according to the antigenic material characteristics. Deposits (thinely particulate material) appeared more frequently, confirming the immunohistochemistry sensitivity to detect small amounts of antigens even when this material is not detected by histochemical stainings.
PY - 1995 SP - 7 EP - 12 T2 - Revista do Instituto de Medicina Tropical de Sao Paulo TI - Pattern of mycobacterial antigen detection in leprosy. VL - 37 SN - 0036-4665 ER -