TY - JOUR
KW - Animals
KW - Antibodies, Monoclonal
KW - Antigens, Bacterial
KW - Armadillos
KW - Biopsy
KW - Epitopes
KW - Humans
KW - Immunoenzyme Techniques
KW - leprosy
KW - Mycobacterium Infections
KW - Mycobacterium leprae
KW - Mycobacterium lepraemurium
KW - Rats
KW - Serial Passage
KW - Skin
AU - Diachin M N
AU - Iushchenko A A
AU - Ibragimov CD
AU - Chernousova L N
AU - Litvinov V I
AB - <p>The antigenic preparations of M. leprae isolated from the biopsy leproma material of leprosy patients, M. leprae obtained by passage through laboratory animals (armadillos, rats) and M. lepraemurium have been identified by indirect solid-phase enzyme immunoassay. Experiments made with the use of the set of monoclonal antibodies (McAb) to different M. leprae antigenic determinants, supplied by the WHO Bank of Monoclonal Antibodies (IMMLEP), have demonstrated the presence of common antigenic determinants in 65-kD protein of M. leprae obtained by passage through armadillos and rats and M. lepraemurium, as well as in 12-kD protein of M. leprae isolated from armadillos and rats and M. leprae isolated from the biotic material of human lepromas. The passage of M. leprae through experimental animals leads to the appearance of a new antigenic determinant in their structure, which is recognized by McAb 111E4.</p>
BT - Zhurnal mikrobiologii, epidemiologii, i immunobiologii
C1 - http://www.ncbi.nlm.nih.gov/pubmed/7514328?dopt=Abstract
DA - 1994 Jan-Feb
IS - 1
J2 - Zh. Mikrobiol. Epidemiol. Immunobiol.
LA - rus
N2 - <p>The antigenic preparations of M. leprae isolated from the biopsy leproma material of leprosy patients, M. leprae obtained by passage through laboratory animals (armadillos, rats) and M. lepraemurium have been identified by indirect solid-phase enzyme immunoassay. Experiments made with the use of the set of monoclonal antibodies (McAb) to different M. leprae antigenic determinants, supplied by the WHO Bank of Monoclonal Antibodies (IMMLEP), have demonstrated the presence of common antigenic determinants in 65-kD protein of M. leprae obtained by passage through armadillos and rats and M. lepraemurium, as well as in 12-kD protein of M. leprae isolated from armadillos and rats and M. leprae isolated from the biotic material of human lepromas. The passage of M. leprae through experimental animals leads to the appearance of a new antigenic determinant in their structure, which is recognized by McAb 111E4.</p>
PY - 1994
SP - 73
EP - 6
T2 - Zhurnal mikrobiologii, epidemiologii, i immunobiologii
TI - [The identification of the antigenic determinants of Mycobacterium leprae passed through laboratory animals by using monoclonal antibodies].
SN - 0372-9311
ER -