TY - JOUR
KW - Base Sequence
KW - DNA Primers
KW - Evaluation Studies as Topic
KW - Humans
KW - leprosy
KW - Molecular Sequence Data
KW - Mycobacterium leprae
KW - polymerase chain reaction
KW - RNA, Bacterial
KW - RNA, Ribosomal, 16S
KW - Species Specificity
AU - Richter E
AU - Duchrow M
AU - Schlüter C
AU - Hahn M
AU - Flad H D
AU - Gerdes J
AB - <p>Recently, polymerase chain reaction has been introduced for the species-specific assessment of Mycobacterium leprae (1). To avoid Southern blotting techniques using radioactively labelled oligonucleotide probes, the aim of this study was to establish a three primer-based single-step PCR technique. Using primers designed for this purpose we amplified a part of the gene encoding for the 16S ribosomal RNA of slowly growing mycobacteria. Due to the species-specific antisense primer a second, smaller fragment specific for M. leprae was amplified. Our results show that the employment of a second antisense primer in the PCR may be a substitution for Southern blot hybridization.</p>
BT - Immunobiology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/7536184?dopt=Abstract
DA - 1994 Oct
DO - 10.1016/S0171-2985(11)80440-7
IS - 4-5
J2 - Immunobiology
LA - eng
N2 - <p>Recently, polymerase chain reaction has been introduced for the species-specific assessment of Mycobacterium leprae (1). To avoid Southern blotting techniques using radioactively labelled oligonucleotide probes, the aim of this study was to establish a three primer-based single-step PCR technique. Using primers designed for this purpose we amplified a part of the gene encoding for the 16S ribosomal RNA of slowly growing mycobacteria. Due to the species-specific antisense primer a second, smaller fragment specific for M. leprae was amplified. Our results show that the employment of a second antisense primer in the PCR may be a substitution for Southern blot hybridization.</p>
PY - 1994
SP - 351
EP - 3
T2 - Immunobiology
TI - Detection of Mycobacterium leprae by three-primer PCR.
VL - 191
SN - 0171-2985
ER -