TY - JOUR
KW - Adult
KW - Animals
KW - Biological Assay
KW - Drug Therapy, Combination
KW - Evaluation Studies as Topic
KW - Humans
KW - Leprostatic Agents
KW - leprosy
KW - Luminescent Measurements
KW - Mice
KW - Middle Aged
KW - Mycobacterium leprae
KW - Time Factors
AU - Gupta U D
AU - Katoch K
AU - Singh H B
AU - Natrajan M
AU - Sharma V D
AU - Katoch V M
AB - <p>Cutaneous biopsies were collected from multibacillary leprosy patients who attended the out-patient department of Jalma Institute for treatment at different time intervals, i.e. 6 months, 12 months, 18 months, 24 months, 30 months, 36 months and 42 months after starting multidrug therapy (MDT) when they were still skin smear positive. Biopsies were processed for inoculation into mouse foot pad (MFP) and estimation of bacillary ATP levels by bioluminescent assay (ATP assay) by earlier established procedures. Viable bacilli were detectable after 1 year (25% cases by MFP and 31% cases by ATP assay), 2 years (8% cases by MFP and 12% cases by ATP assay) and 3 years (4% cases by both MFP and ATP assays). Overall, the percentage of the persisters was 10% by MFP and 13% by ATP assay. It would be important to carry out surveillance studies in larger number of BL/LL cases to know the trends and also the resultant relapses.</p>
BT - Acta leprologica
C1 - http://www.ncbi.nlm.nih.gov/pubmed/10544720?dopt=Abstract
DA - 1999
IS - 3
J2 - Acta Leprol
LA - eng
N2 - <p>Cutaneous biopsies were collected from multibacillary leprosy patients who attended the out-patient department of Jalma Institute for treatment at different time intervals, i.e. 6 months, 12 months, 18 months, 24 months, 30 months, 36 months and 42 months after starting multidrug therapy (MDT) when they were still skin smear positive. Biopsies were processed for inoculation into mouse foot pad (MFP) and estimation of bacillary ATP levels by bioluminescent assay (ATP assay) by earlier established procedures. Viable bacilli were detectable after 1 year (25% cases by MFP and 31% cases by ATP assay), 2 years (8% cases by MFP and 12% cases by ATP assay) and 3 years (4% cases by both MFP and ATP assays). Overall, the percentage of the persisters was 10% by MFP and 13% by ATP assay. It would be important to carry out surveillance studies in larger number of BL/LL cases to know the trends and also the resultant relapses.</p>
PY - 1999
SP - 89
EP - 92
T2 - Acta leprologica
TI - Detection of viable organisms in leprosy patients treated with multidrug therapy.
VL - 11
SN - 0001-5938
ER -