The cell walls of 24 coryneform non-acid-fast, Gram-positive organisms isolated from human leprosy lesion, were hydrolysed and analysed. Four known chemical markers of different high polymer components of the walls of microorganisms of the CMN (Corynebacterium, Mycobacterium, Nocardia) group were detected in whole cells and cell wall hydrolysates of the coryneform bacteria analyzed. These markers were: meso-diaminopimelic acid (peptidoglycan), arabinose and galactose (arabinogalactan), and mycolic acids. In addition, mycolic acids proved to be of the corynomycolic type, as shown by thin layer chromatography analysis. The conclusion was drawn that these coryneform strains independently isolated from patients of different countries, represent a homogeneous group within the genus Corynebacterium. This inference is supported by a parallel work showing that the guanine-plus-cytosine content of the DNA of these coryneform strains falls within the range of values characteristic of true corynebacteria pathogenic for animals.
BT - Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious... C1 - http://www.ncbi.nlm.nih.gov/pubmed/7199235?dopt=Abstract DA - 1981 Dec IS - 1 J2 - Zentralbl Bakteriol Mikrobiol Hyg A LA - eng N2 -The cell walls of 24 coryneform non-acid-fast, Gram-positive organisms isolated from human leprosy lesion, were hydrolysed and analysed. Four known chemical markers of different high polymer components of the walls of microorganisms of the CMN (Corynebacterium, Mycobacterium, Nocardia) group were detected in whole cells and cell wall hydrolysates of the coryneform bacteria analyzed. These markers were: meso-diaminopimelic acid (peptidoglycan), arabinose and galactose (arabinogalactan), and mycolic acids. In addition, mycolic acids proved to be of the corynomycolic type, as shown by thin layer chromatography analysis. The conclusion was drawn that these coryneform strains independently isolated from patients of different countries, represent a homogeneous group within the genus Corynebacterium. This inference is supported by a parallel work showing that the guanine-plus-cytosine content of the DNA of these coryneform strains falls within the range of values characteristic of true corynebacteria pathogenic for animals.
PY - 1981 SP - 114 EP - 25 T2 - Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious... TI - Chemical identification of some cell-wall components of microorganisms isolated from human leprosy lesions. VL - 251 SN - 0174-3031 ER -