LDH isoenzymes were studied in tissue extracts of 78 cases of leprosy. All 25 control tissues showed five LDH isoenzymes corresponding to those of human sera. All tissues from the leprosy cases showed five similar bands. Seventeen cases showed additional LDH isoenzymes (anomalous bands). In 12 cases there was a single extra band with an eF value of either 0.125 +/- 0.015 or 0.525 +/- 0.015, and five cases showed both these bands. Additional bands were observed only in cases positive for acid-fast microorganisms (17 of 27 cases), and their presence correlated well with bacterial load (as judged by the BI) and viable organisms (as judged by the MI). Four cases with a high BI and MI did not show anomalous bands, however. A plausible explanation for these bands is that they originate from viable M. leprae.
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 -http://www.ncbi.nlm.nih.gov/pubmed/7193657?dopt=Abstract
DA - 1980 Dec IS - 4 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -LDH isoenzymes were studied in tissue extracts of 78 cases of leprosy. All 25 control tissues showed five LDH isoenzymes corresponding to those of human sera. All tissues from the leprosy cases showed five similar bands. Seventeen cases showed additional LDH isoenzymes (anomalous bands). In 12 cases there was a single extra band with an eF value of either 0.125 +/- 0.015 or 0.525 +/- 0.015, and five cases showed both these bands. Additional bands were observed only in cases positive for acid-fast microorganisms (17 of 27 cases), and their presence correlated well with bacterial load (as judged by the BI) and viable organisms (as judged by the MI). Four cases with a high BI and MI did not show anomalous bands, however. A plausible explanation for these bands is that they originate from viable M. leprae.
PY - 1980 SP - 426 EP - 30 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - Lactate dehydrogenase zymograms of skin biopsies in patients with leprosy. A preliminary report. UR - http://ila.ilsl.br/pdfs/v48n4a09.pdf VL - 48 SN - 0148-916X ER -