TY - JOUR
KW - Adolescent
KW - Adult
KW - Aged
KW - Antibodies, Bacterial
KW - Antigens, Bacterial
KW - Blood Specimen Collection
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Glycolipids
KW - Humans
KW - leprosy
KW - Linear Models
KW - Male
KW - Middle Aged
KW - Mycobacterium leprae
AU - Tomimori-Yamashita J
AU - Nguyen T H
AU - Maeda S M
AU - Flageul B
AU - Rotta O
AU - Cruaud P
AB - <p>The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I) from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS) containing 0.5% gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals.</p>
BT - Revista do Instituto de Medicina Tropical de Sao Paulo
C1 - http://www.ncbi.nlm.nih.gov/pubmed/10564918?dopt=Abstract
DA - 1999 Jul-Aug
DO - 10.1590/s0036-46651999000400007
IS - 4
J2 - Rev. Inst. Med. Trop. Sao Paulo
LA - eng
N2 - <p>The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I) from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS) containing 0.5% gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals.</p>
PY - 1999
SP - 239
EP - 42
T2 - Revista do Instituto de Medicina Tropical de Sao Paulo
TI - Anti-phenolic glycolipid-I (PGL-I) determination using blood collection on filter paper in leprosy patients.
UR - http://www.scielo.br/pdf/rimtsp/v41n4/v41n4a08.pdf
VL - 41
SN - 0036-4665
ER -