To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The results show that anti-M. leprae antibodies were synthesized in vitro in the biopsies from the skin lesions of leprosy patients and that the specificity of the locally produced antibodies varied from patient to patient.
BT - Infection and immunity C1 - http://www.ncbi.nlm.nih.gov/pubmed/6991410?dopt=Abstract DA - 1980 Feb IS - 2 J2 - Infect. Immun. LA - eng N2 -To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The results show that anti-M. leprae antibodies were synthesized in vitro in the biopsies from the skin lesions of leprosy patients and that the specificity of the locally produced antibodies varied from patient to patient.
PY - 1980 SP - 297 EP - 301 T2 - Infection and immunity TI - In vitro synthesis of anti-mycobacterial antibodies in biopsies from skin lesions of leprosy patients. UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC550764/pdf/iai00170-0015.pdf VL - 27 SN - 0019-9567 ER -