A modified allochrome staining procedure is presented as being the most reliable and sensitive method for demonstrating mycobacteria in tissue sections. The technic is as follows: Deparaffinize formalin fixed sections, oxidize in 10% periodic acid for 24 hours, differentiate in 1% HCl-70% ethanol, stain in Weigert's iron hematoxylin nuclear stain, and counterstain in picro-methyl glue. Mycobacteria stained brilliant red in contrast with the allochrome-stained background tissues, and apparently otherwise chromophobic bacilli are demonstrated.
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 -http://www.ncbi.nlm.nih.gov/pubmed/68940?dopt=Abstract
DA - 1977 Jan-Mar IS - 1 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -A modified allochrome staining procedure is presented as being the most reliable and sensitive method for demonstrating mycobacteria in tissue sections. The technic is as follows: Deparaffinize formalin fixed sections, oxidize in 10% periodic acid for 24 hours, differentiate in 1% HCl-70% ethanol, stain in Weigert's iron hematoxylin nuclear stain, and counterstain in picro-methyl glue. Mycobacteria stained brilliant red in contrast with the allochrome-stained background tissues, and apparently otherwise chromophobic bacilli are demonstrated.
PY - 1977 SP - 49 EP - 51 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - A modified allochrome procedure for demonstrating mycobacteria in tissue sections. UR - http://ila.ilsl.br/pdfs/v45n1a07.pdf VL - 45 SN - 0148-916X ER -