DNA derived from Mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. The genome size and guanine-plus-cytosine content of M. leprae were 1.3 X 10(9) and 55.8%, respectively. Among selected strains of mycobacterial, nocardial and corynebacterial species, Corynebacterium sp. 2628 LB, isolated from a human leprosy patient, showed the highest DNA homology with M. leprae. Of the DNAs derived from mycobacteria, those of M. tuberculosis and M. scrofulaceum showed a comparatively high reassociation with the DNA of M. leprae.
BT - Leprosy in India C1 - http://www.ncbi.nlm.nih.gov/pubmed/6764515?dopt=Abstract CN - Infolep Library - available DA - 1982 Oct IS - 4 J2 - Lepr India LA - eng N2 -DNA derived from Mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. The genome size and guanine-plus-cytosine content of M. leprae were 1.3 X 10(9) and 55.8%, respectively. Among selected strains of mycobacterial, nocardial and corynebacterial species, Corynebacterium sp. 2628 LB, isolated from a human leprosy patient, showed the highest DNA homology with M. leprae. Of the DNAs derived from mycobacteria, those of M. tuberculosis and M. scrofulaceum showed a comparatively high reassociation with the DNA of M. leprae.
PY - 1982 SP - 801 EP - 8 T2 - Leprosy in India TI - DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation. VL - 54 SN - 0024-1024 ER -