A purified preparation of M. leprae antigen 7 was used to investigate the specificity of anti-M. Leprae 7 antibodies in leprosy sera in a radioimmunoassay. A solution containing arabinogalactan (AG) and arabinomannan (AM) inhibited the antibodies in some sera to a great extent, whereas the antibody activity was virtually unchanged in other sera under the same conditions. These findings indicate that the antibodies are directed against different determinants on M. leprae antigen 7. Antibodies against determinants other than AG and AM occurred particularly in lepromatous leprosy sera. In 12 out of 14 sera, AG and AM had similar inhibiting capacity. In one serum, AG had markedly higher inhibiting capacity than AM; in the last serum, the reverse was the case, demonstrating variation in anti-polysaccharide specificity in individual sera.
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 - http://www.ncbi.nlm.nih.gov/pubmed/6168595?dopt=Abstract DA - 1981 Jun IS - 2 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -A purified preparation of M. leprae antigen 7 was used to investigate the specificity of anti-M. Leprae 7 antibodies in leprosy sera in a radioimmunoassay. A solution containing arabinogalactan (AG) and arabinomannan (AM) inhibited the antibodies in some sera to a great extent, whereas the antibody activity was virtually unchanged in other sera under the same conditions. These findings indicate that the antibodies are directed against different determinants on M. leprae antigen 7. Antibodies against determinants other than AG and AM occurred particularly in lepromatous leprosy sera. In 12 out of 14 sera, AG and AM had similar inhibiting capacity. In one serum, AG had markedly higher inhibiting capacity than AM; in the last serum, the reverse was the case, demonstrating variation in anti-polysaccharide specificity in individual sera.
PY - 1981 SP - 147 EP - 58 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - Demonstration of antibodies reacting with different determinants on Mycobacterium leprae antigen 7. VL - 49 SN - 0148-916X ER -