TY - JOUR
KW - Cytokines
KW - Dose-Response Relationship, Drug
KW - Humans
KW - Interferon-gamma
KW - Interleukin-12
KW - Interleukin-18
KW - Killer Cells, Natural
KW - leprosy
KW - Monocytes
KW - T-Lymphocytes
KW - Tuberculosis, Pulmonary
AU - García V E
AU - Uyemura K
AU - Sieling P A
AU - Ochoa M T
AU - Morita C T
AU - Okamura H
AU - Kurimoto M
AU - Rea T H
AU - Modlin R L
AB - <p>We investigated the role of IL-18 in leprosy, a disease characterized by polar cytokine responses that correlate with clinical disease. In vivo, IL-18 mRNA expression was higher in lesions from resistant tuberculoid as compared with susceptible lepromatous patients, and, in vitro, monocytes produced IL-18 in response to Mycobacterium leprae. rIL-18 augmented M. leprae-induced IFN-gamma in tuberculoid patients, but not lepromatous patients, while IL-4 production was not induced by IL-18. Anti-IL-12 partially inhibited M. leprae-induced release of IFN-gamma in the presence of IL-18, suggesting a combined effect of IL-12 and IL-18 in promoting M. leprae-specific type 1 responses. IL-18 enhanced M. leprae-induced IFN-gamma production rapidly (24 h) by NK cells and in a more sustained manner (5 days) by T cells. Finally, IL-18 directly induced IFN-gamma production from mycobacteria-reactive T cell clones. These results suggest that IL-18 induces type 1 cytokine responses in the host defense against intracellular infection.</p>
BT - Journal of immunology (Baltimore, Md. : 1950)
C1 - http://www.ncbi.nlm.nih.gov/pubmed/10229854?dopt=Abstract
DA - 1999 May 15
IS - 10
J2 - J. Immunol.
LA - eng
N2 - <p>We investigated the role of IL-18 in leprosy, a disease characterized by polar cytokine responses that correlate with clinical disease. In vivo, IL-18 mRNA expression was higher in lesions from resistant tuberculoid as compared with susceptible lepromatous patients, and, in vitro, monocytes produced IL-18 in response to Mycobacterium leprae. rIL-18 augmented M. leprae-induced IFN-gamma in tuberculoid patients, but not lepromatous patients, while IL-4 production was not induced by IL-18. Anti-IL-12 partially inhibited M. leprae-induced release of IFN-gamma in the presence of IL-18, suggesting a combined effect of IL-12 and IL-18 in promoting M. leprae-specific type 1 responses. IL-18 enhanced M. leprae-induced IFN-gamma production rapidly (24 h) by NK cells and in a more sustained manner (5 days) by T cells. Finally, IL-18 directly induced IFN-gamma production from mycobacteria-reactive T cell clones. These results suggest that IL-18 induces type 1 cytokine responses in the host defense against intracellular infection.</p>
PY - 1999
SP - 6114
EP - 21
T2 - Journal of immunology (Baltimore, Md. : 1950)
TI - IL-18 promotes type 1 cytokine production from NK cells and T cells in human intracellular infection.
UR - http://www.jimmunol.org/content/162/10/6114.full.pdf+html
VL - 162
SN - 0022-1767
ER -