TY - JOUR KW - Animals KW - Antibodies, Protozoan KW - Antibody Specificity KW - Blotting, Western KW - Case-Control Studies KW - Cross Reactions KW - Evaluation Studies as Topic KW - Humans KW - India KW - Leishmania donovani KW - Leishmaniasis, Visceral KW - Serologic Tests AU - Tiwari V AU - Jain A AU - Agarwal M AU - Mittal B AU - Pandey H P AB -

When infected with Leishmania species, patients develop specific antibodies that constitute the basis of serodiagnosis. using Western blot analysis we studied the specificity of anti-leishmania donovani antibodies in patients with visceral leishmaniasis, healthy subjects living in an endemic and non-endemic areas, and patients of other infectious diseases like malaria, leprosy, tuberculosis and tropical splenomegaly. Sera from patients with kala-azar recognised numerous antigens that had a molecular weight of 150 KD, 145 KD, 120 KD, 92 KD, 87 KD, 72 KD, 65 KD, 56 KD, 50 KD, 40 KD, 26 KD, 21 KD, 14 KD, AND 12 KD. The 150, 145, 120, 92, 87, 81, 65, 25, 21, 14, and 12 KD antigens had the greatest specificity for kala-azar sera while the bands of molecular weights 72, 56, 50, and 40 KD were found to be cross reactive with sera of patients of other diseases.

BT - Biochemistry and molecular biology international C1 - http://www.ncbi.nlm.nih.gov/pubmed/8624503?dopt=Abstract DA - 1995 Nov IS - 5 J2 - Biochem. Mol. Biol. Int. LA - eng N2 -

When infected with Leishmania species, patients develop specific antibodies that constitute the basis of serodiagnosis. using Western blot analysis we studied the specificity of anti-leishmania donovani antibodies in patients with visceral leishmaniasis, healthy subjects living in an endemic and non-endemic areas, and patients of other infectious diseases like malaria, leprosy, tuberculosis and tropical splenomegaly. Sera from patients with kala-azar recognised numerous antigens that had a molecular weight of 150 KD, 145 KD, 120 KD, 92 KD, 87 KD, 72 KD, 65 KD, 56 KD, 50 KD, 40 KD, 26 KD, 21 KD, 14 KD, AND 12 KD. The 150, 145, 120, 92, 87, 81, 65, 25, 21, 14, and 12 KD antigens had the greatest specificity for kala-azar sera while the bands of molecular weights 72, 56, 50, and 40 KD were found to be cross reactive with sera of patients of other diseases.

PY - 1995 SP - 959 EP - 64 T2 - Biochemistry and molecular biology international TI - Evaluation of antibody responses in Indian kala-azar by immunoblot. VL - 37 SN - 1039-9712 ER -