Polysulfone membranes have been used as a solid support for chromatography and immunoblotting of phenolic glycolipid I from Mycobacterium leprae. These membranes have an advantage over other supports such as nitrocellulose and silica gel in that very little non-specific background binding of antibodies occurs and assays can readily be carried out with IgM antibodies from human sera. An example of use of the polysulfone chromatography system for detection of phenolic glycolipid I in sera from leprosy patients is described.
BT - Journal of immunological methods C1 - http://www.ncbi.nlm.nih.gov/pubmed/3889165?dopt=Abstract DA - 1985 May 23 DO - 10.1016/0022-1759(85)90100-0 IS - 2 J2 - J. Immunol. Methods LA - eng N2 -Polysulfone membranes have been used as a solid support for chromatography and immunoblotting of phenolic glycolipid I from Mycobacterium leprae. These membranes have an advantage over other supports such as nitrocellulose and silica gel in that very little non-specific background binding of antibodies occurs and assays can readily be carried out with IgM antibodies from human sera. An example of use of the polysulfone chromatography system for detection of phenolic glycolipid I in sera from leprosy patients is described.
PY - 1985 SP - 205 EP - 11 T2 - Journal of immunological methods TI - Use of a polysulfone membrane support for immunochemical analysis of a glycolipid from Mycobacterium leprae. VL - 79 SN - 0022-1759 ER -