TY - JOUR KW - Adolescent KW - Adult KW - Animals KW - Armadillos KW - BCG Vaccine KW - Dose-Response Relationship, Immunologic KW - Humans KW - leprosy KW - Lymphocyte Activation KW - Middle Aged KW - Mycobacterium bovis KW - Mycobacterium leprae KW - Tuberculin AU - Smelt A H AU - Liew F Y AU - Rees R J AB -

The lymphocyte transformation test was applied to compare in vitro lymphocyte responses of tuberculoid (high resistant) and lepromatous (low resistant) leprosy patients to purified Mycobacterium leprae derived from experimentally infected armadillos and crude M. leprae derived from man, as well as to bacille Calmette-Guérin (BCG) and purified protein derivative (PPD). It was found that the purification procedure using enzymic digestion did not affect the immunogenicity of armadillo-derived M. leprae as compared with the crude human-derived preparation, although 2.5-5-fold higher doses of the purified organisms were required to elicitate equivalent lymphocyte responses. The result indicated the suitability of purified armadillo-derived M. leprae as the standard antigen for lymphocytes transformation tests in leprosy. The cross-reactivity studies show a close relationship between PPD and BCG, but not between M. leprae and PPD or BCG.

BT - Clinical and experimental immunology C1 - http://www.ncbi.nlm.nih.gov/pubmed/367652?dopt=Abstract DA - 1978 Nov IS - 2 J2 - Clin. Exp. Immunol. LA - eng N2 -

The lymphocyte transformation test was applied to compare in vitro lymphocyte responses of tuberculoid (high resistant) and lepromatous (low resistant) leprosy patients to purified Mycobacterium leprae derived from experimentally infected armadillos and crude M. leprae derived from man, as well as to bacille Calmette-Guérin (BCG) and purified protein derivative (PPD). It was found that the purification procedure using enzymic digestion did not affect the immunogenicity of armadillo-derived M. leprae as compared with the crude human-derived preparation, although 2.5-5-fold higher doses of the purified organisms were required to elicitate equivalent lymphocyte responses. The result indicated the suitability of purified armadillo-derived M. leprae as the standard antigen for lymphocytes transformation tests in leprosy. The cross-reactivity studies show a close relationship between PPD and BCG, but not between M. leprae and PPD or BCG.

PY - 1978 SP - 164 EP - 9 T2 - Clinical and experimental immunology TI - Lymphocyte response of leprosy patients to human-derived and purified armadillo-derived Mycobacterium leprae, BCG and PPD. VL - 34 SN - 0009-9104 ER -