The ultrastructural changes that develop in mouse peritoneal macrophages from 10 minutes up to 14 weeks after exposure to Mycobacterium leprae are presented. Phagocytosis occurred by a process of engulfment by cytoplasmic processes and incorporation into a phagosome, into which lysosomal enzymes were subsequently introduced. Electron transparent zones (E.T.Z.) were not observed around phagocytosed bacilli in this study, however discrete droplets of lipid-like material appeared in the cytoplasm of macrophages, between 2 and 4 weeks after ingestion of the micro-organisms. Phagosomes with double limiting membranes were observed in macrophages harvested as early as 40 minutes after exposure to M. leprae, contrary to the observations of Evans and Levy (1972).
BT - Leprosy in India C1 - http://www.ncbi.nlm.nih.gov/pubmed/359923?dopt=Abstract CN - Infolep Library - available DA - 1978 Jul IS - 3 J2 - Lepr India LA - eng N2 -The ultrastructural changes that develop in mouse peritoneal macrophages from 10 minutes up to 14 weeks after exposure to Mycobacterium leprae are presented. Phagocytosis occurred by a process of engulfment by cytoplasmic processes and incorporation into a phagosome, into which lysosomal enzymes were subsequently introduced. Electron transparent zones (E.T.Z.) were not observed around phagocytosed bacilli in this study, however discrete droplets of lipid-like material appeared in the cytoplasm of macrophages, between 2 and 4 weeks after ingestion of the micro-organisms. Phagosomes with double limiting membranes were observed in macrophages harvested as early as 40 minutes after exposure to M. leprae, contrary to the observations of Evans and Levy (1972).
PY - 1978 SP - 345 EP - 57 T2 - Leprosy in India TI - The early cellular response to M. leprae. An ultrastructural study. VL - 50 SN - 0024-1024 ER -