TY - JOUR
KW - Animals
KW - Antibodies, Bacterial
KW - Antibody Formation
KW - Antigens, Bacterial
KW - Armadillos
KW - Enzyme-Linked Immunosorbent Assay
KW - Glycolipids
KW - leprosy
KW - Mycobacterium leprae
KW - Time Factors
KW - Xenarthra
AU - Truman R W
AU - Morales M J
AU - Shannon E J
AU - Hastings R C
AB - <p>An enzyme-linked immunosorbent assay (ELISA) for IgM antibodies to the phenolic glycolipid-I antigen of Mycobacterium leprae was evaluated for efficacy in monitoring armadillos experimentally infected with the bacillus. IgM antibodies were detected in armadillos from 186 days after experimental infection until the animals were sacrificed. The ELISA demonstrated the establishment of infection earlier and more reliably than the histologic methods previously applied. Satisfactory yields of M. leprae from individual armadillos could be predicted 97% of the time, and the technique may be useful in identifying appropriate harvest times or resistance among armadillos. The ELISA seems to be a valuable adjunct for managing experimental infections of M. leprae in armadillos.</p>
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
C1 - http://www.ncbi.nlm.nih.gov/pubmed/3546545?dopt=Abstract
DA - 1986 Dec
IS - 4
J2 - Int. J. Lepr. Other Mycobact. Dis.
LA - eng
N2 - <p>An enzyme-linked immunosorbent assay (ELISA) for IgM antibodies to the phenolic glycolipid-I antigen of Mycobacterium leprae was evaluated for efficacy in monitoring armadillos experimentally infected with the bacillus. IgM antibodies were detected in armadillos from 186 days after experimental infection until the animals were sacrificed. The ELISA demonstrated the establishment of infection earlier and more reliably than the histologic methods previously applied. Satisfactory yields of M. leprae from individual armadillos could be predicted 97% of the time, and the technique may be useful in identifying appropriate harvest times or resistance among armadillos. The ELISA seems to be a valuable adjunct for managing experimental infections of M. leprae in armadillos.</p>
PY - 1986
SP - 556
EP - 9
T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
TI - Evaluation of monitoring antibodies to PGL-I in armadillos experimentally infected with M. leprae.
VL - 54
SN - 0148-916X
ER -