Two methods for detecting the phenolic glycolipid, PGL-1, a Mycobacterium leprae-specific molecule, in the urine of leprosy patients are described. Both methods rely on the 100-fold preconcentration of the urine, which can be accomplished by a single-step ultrafiltration procedure. The equivalent of approximately 2.5 micrograms of PGL-1/ml was detected in the urine of LL patients with an inhibition ELISA. The second method, a direct dot-blot assay on nitrocellulose paper, was much simpler and more sensitive. As little as 3 ng of antigen was detected by the dot-blot technique. PGL-1 was detected in the urine of LL patients.
BT - Scandinavian journal of immunology C1 - http://www.ncbi.nlm.nih.gov/pubmed/3544198?dopt=Abstract DA - 1987 Jan DO - 10.1111/j.1365-3083.1987.tb01044.x IS - 1 J2 - Scand. J. Immunol. LA - eng N2 -Two methods for detecting the phenolic glycolipid, PGL-1, a Mycobacterium leprae-specific molecule, in the urine of leprosy patients are described. Both methods rely on the 100-fold preconcentration of the urine, which can be accomplished by a single-step ultrafiltration procedure. The equivalent of approximately 2.5 micrograms of PGL-1/ml was detected in the urine of LL patients with an inhibition ELISA. The second method, a direct dot-blot assay on nitrocellulose paper, was much simpler and more sensitive. As little as 3 ng of antigen was detected by the dot-blot technique. PGL-1 was detected in the urine of LL patients.
PY - 1987 SP - 37 EP - 43 T2 - Scandinavian journal of immunology TI - Methods for the detection of a specific Mycobacterium leprae antigen in the urine of leprosy patients. VL - 25 SN - 0300-9475 ER -