A killed, armadillo-derived Mycobacterium leprae vaccine was examined for its ability to induce cell-mediated responsiveness in purified protein derivative (PPD)-positive volunteers residing in a nonendemic country using the lymphocyte transformation test (LTT). A marked increase in the proliferative responses to a M. leprae-soluble antigen preparation was observed in the two groups which were vaccinated with the highest doses of the vaccine, i.e., 1.5 X 10(8) and 5 X 10(8) bacilli. This increase was observed in both groups 3 months after vaccination, and persisted for the study period of 1 year. The in vitro proliferative responses to whole bacilli, of both armadillo and human origin, showed a similar but smaller increase 3 months after vaccination. Some enhancement of responses to cross-reactive antigens, such as PPD, and to unrelated antigens such as streptokinase-streptodornase, tetanus toxoid and diphtheria toxoid, was also observed. Thus, the LTT revealed that while the killed M. leprae vaccine induced a specific cell-mediated response to M. leprae, it was also responsible for a nonspecific immune-enhancement effect in healthy volunteers.
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 - http://www.ncbi.nlm.nih.gov/pubmed/3549938?dopt=Abstract DA - 1987 Mar IS - 1 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -A killed, armadillo-derived Mycobacterium leprae vaccine was examined for its ability to induce cell-mediated responsiveness in purified protein derivative (PPD)-positive volunteers residing in a nonendemic country using the lymphocyte transformation test (LTT). A marked increase in the proliferative responses to a M. leprae-soluble antigen preparation was observed in the two groups which were vaccinated with the highest doses of the vaccine, i.e., 1.5 X 10(8) and 5 X 10(8) bacilli. This increase was observed in both groups 3 months after vaccination, and persisted for the study period of 1 year. The in vitro proliferative responses to whole bacilli, of both armadillo and human origin, showed a similar but smaller increase 3 months after vaccination. Some enhancement of responses to cross-reactive antigens, such as PPD, and to unrelated antigens such as streptokinase-streptodornase, tetanus toxoid and diphtheria toxoid, was also observed. Thus, the LTT revealed that while the killed M. leprae vaccine induced a specific cell-mediated response to M. leprae, it was also responsible for a nonspecific immune-enhancement effect in healthy volunteers.
PY - 1987 SP - 30 EP - 5 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - In vitro proliferation of lymphocytes from human volunteers vaccinated with armadillo-derived, killed M. leprae. VL - 55 SN - 0148-916X ER -