TY - JOUR
KW - Antigens, Bacterial
KW - Histocompatibility Antigens Class II
KW - Humans
KW - Hypersensitivity, Delayed
KW - Langerhans Cells
KW - leprosy
KW - Mycobacterium leprae
KW - Neutrophils
KW - T-Lymphocytes
AU - Narayanan R B
AU - Ramu G
AU - Malaviya G N
AU - Sinha S
AU - Sengupta U
AB - <p>A study was made on the in situ characteristics of dermal infiltrates in a 24-hr skin reaction using monoclonal antibodies defining T-cell subsets, Ia-like antigens, Langerhans' cells, My1, and indirect immunofluorescence. The skin reaction was induced by the mycobacterial antigen My1 derived from Mycobacterium leprae. In all, 10 biopsies were studied. The infiltrates were composed of lymphocytes and polymorphonuclear leukocytes. The predominant lymphocytes in the infiltrates were activated T lymphocytes expressing OKT11, Leu3a, OKT8, and Ia-like antigens. The ratio of Leu3a+:OKT8+ cells was 1.2 +/- 0.50. Some of the cells in the infiltrates showed the presence of My1, as seen by the staining with MLO6 monoclonal antibody. No difference was observed in the numbers of OKT6+ epidermal Langerhans' cells in the skin of My1 reaction biopsies and those of normal individuals.</p>
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
C1 - http://www.ncbi.nlm.nih.gov/pubmed/3519804?dopt=Abstract
DA - 1986 Mar
IS - 1
J2 - Int. J. Lepr. Other Mycobact. Dis.
LA - eng
N2 - <p>A study was made on the in situ characteristics of dermal infiltrates in a 24-hr skin reaction using monoclonal antibodies defining T-cell subsets, Ia-like antigens, Langerhans' cells, My1, and indirect immunofluorescence. The skin reaction was induced by the mycobacterial antigen My1 derived from Mycobacterium leprae. In all, 10 biopsies were studied. The infiltrates were composed of lymphocytes and polymorphonuclear leukocytes. The predominant lymphocytes in the infiltrates were activated T lymphocytes expressing OKT11, Leu3a, OKT8, and Ia-like antigens. The ratio of Leu3a+:OKT8+ cells was 1.2 +/- 0.50. Some of the cells in the infiltrates showed the presence of My1, as seen by the staining with MLO6 monoclonal antibody. No difference was observed in the numbers of OKT6+ epidermal Langerhans' cells in the skin of My1 reaction biopsies and those of normal individuals.</p>
PY - 1986
SP - 46
EP - 51
T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
TI - Immunohistological analysis of skin reaction to My1 derived from Mycobacterium leprae.
VL - 54
SN - 0148-916X
ER -