Immunoblotting has provided a powerful and effective approach to dissection of the immune response to mycobacterial antigens in a situation in which the availability of isolated antigenic components is severely limited. The basic approach of blotting on to a solid-phase support has been used in combination with SDS/polyacrylamide-gel electrophoresis, t.l.c., recombinant DNA technology and T-cell cloning in order to carry out a comprehensive analysis of glycolipid and protein antigens involved in the immune response to mycobacterial infection.
BT - Biochemical Society transactions C1 - http://www.ncbi.nlm.nih.gov/pubmed/3286317?dopt=Abstract DA - 1988 Apr DO - 10.1042/bst0160143 IS - 2 J2 - Biochem. Soc. Trans. LA - eng N2 -Immunoblotting has provided a powerful and effective approach to dissection of the immune response to mycobacterial antigens in a situation in which the availability of isolated antigenic components is severely limited. The basic approach of blotting on to a solid-phase support has been used in combination with SDS/polyacrylamide-gel electrophoresis, t.l.c., recombinant DNA technology and T-cell cloning in order to carry out a comprehensive analysis of glycolipid and protein antigens involved in the immune response to mycobacterial infection.
PY - 1988 SP - 143 EP - 4 T2 - Biochemical Society transactions TI - Immunoblotting and the immune response to leprosy. VL - 16 SN - 0300-5127 ER -