TY - JOUR
KW - Enzyme-Linked Immunosorbent Assay
KW - Epidemiologic Methods
KW - Humans
KW - leprosy
KW - Predictive Value of Tests
KW - Sensitivity and Specificity
AU - Burgess P J
AU - Fine PE
AU - Ponnighaus J M
AU - Draper C
AB - <p>This paper examines the sensitivity and specificity of two ELISA assays for IgM antibodies to Mycobacterium leprae, one employing natural phenolic glycolipid and the other employing a synthetic disaccharide glycoconjugate as antigen. Estimates of sensitivity and specificity are derived, based on a panel of sera from leprosy cases in Malawi and various non-leprosy controls from the UK. Though both assays were able to identify a high proportion of multibacillary patients, neither was able to detect a high proportion of paucibacillary patients without considerable loss of specificity. The implications of the inverse relationship between sensitivity and specificity are discussed with reference to the predictive value of such tests in such areas as Malawi, where the large majority of cases are paucibacillary.</p>
BT - Epidemiology and infection
C1 - http://www.ncbi.nlm.nih.gov/pubmed/3042436?dopt=Abstract
CN - BURGESS1988
DA - 1988 Aug
DO - 10.1017/s0950268800029320
IS - 1
J2 - Epidemiol. Infect.
LA - eng
N2 - <p>This paper examines the sensitivity and specificity of two ELISA assays for IgM antibodies to Mycobacterium leprae, one employing natural phenolic glycolipid and the other employing a synthetic disaccharide glycoconjugate as antigen. Estimates of sensitivity and specificity are derived, based on a panel of sera from leprosy cases in Malawi and various non-leprosy controls from the UK. Though both assays were able to identify a high proportion of multibacillary patients, neither was able to detect a high proportion of paucibacillary patients without considerable loss of specificity. The implications of the inverse relationship between sensitivity and specificity are discussed with reference to the predictive value of such tests in such areas as Malawi, where the large majority of cases are paucibacillary.</p>
PY - 1988
SP - 159
EP - 71
T2 - Epidemiology and infection
TI - Serological tests in leprosy. The sensitivity, specificity and predictive value of ELISA tests based on phenolic glycolipid antigens, and the implications for their use in epidemiological studies.
UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2249338/pdf/epidinfect00010-0161.pdf
VL - 101
SN - 0950-2688
ER -