An enzyme immunoassay (EIA) was developed to detect, in human sera, IgG rheumatoid factor (RF) combining with human IgG. Wells of the EIA plate were coated with the Fc fragment of human IgG. Binding of RF was detected by goat antibodies to F(ab')2 of human IgG followed by rabbit anti-goat IgG conjugated with alkaline phosphatase and finally by the substrate. Using this procedure, RF was detected in 35%-85% of various pathological human sera, but only in 7% of normal human sera. An analogous procedure was also described for detection, in rabbit sera, of an RF combining with rabbit IgG.
BT - Immunological investigations C1 - http://www.ncbi.nlm.nih.gov/pubmed/3069713?dopt=Abstract DA - 1988 Aug-Oct DO - 10.3109/08820138809030589 IS - 6-7 J2 - Immunol. Invest. LA - eng N2 -An enzyme immunoassay (EIA) was developed to detect, in human sera, IgG rheumatoid factor (RF) combining with human IgG. Wells of the EIA plate were coated with the Fc fragment of human IgG. Binding of RF was detected by goat antibodies to F(ab')2 of human IgG followed by rabbit anti-goat IgG conjugated with alkaline phosphatase and finally by the substrate. Using this procedure, RF was detected in 35%-85% of various pathological human sera, but only in 7% of normal human sera. An analogous procedure was also described for detection, in rabbit sera, of an RF combining with rabbit IgG.
PY - 1988 SP - 561 EP - 5 T2 - Immunological investigations TI - Enzyme immunoassay for IgG rheumatoid factor combining with homologous IgG. VL - 17 SN - 0882-0139 ER -