TY - JOUR
KW - Codon
KW - DNA, Bacterial
KW - DNA-Directed RNA Polymerases
KW - Humans
KW - Leprostatic Agents
KW - leprosy
KW - Mutation
KW - Mycobacterium leprae
KW - Plant Proteins
KW - polymerase chain reaction
KW - Rifampin
KW - Sequence Analysis, DNA
KW - Thailand
AU - Ramasoota P
AU - Wongwit W
AU - Sampunachot P
AU - Unnarat K
AU - Ngamying M
AU - Svenson S B
AB - <p>A new finding is reported of multiple mutations in the rpoB gene of 9 Mycobacterium leprae strains from leprosy patients in Thailand, who did not respond to therapy even when rifampicin, the main drug in multi-drug therapy was used. By means of sequence analysis of 9 Thai M. leprae strains, various mutations in 289 bps of the rpoB gene revealed forms of mutation never before described, such as multiple mutations (ie, mutation at two, three, six, seven, eight and nine positions in the rpoB gene), most of which were point-mutation substitutions (a few of which were silent), and some insertions. This investigation demonstrates that mutation in the rpoB gene of M. leprae strains from Thailand involves more variety than previously reported for rpoB mutation patterns in rifampicin resistance M. leprae strains.</p>
BT - The Southeast Asian journal of tropical medicine and public health
C1 - http://www.ncbi.nlm.nih.gov/pubmed/11289008?dopt=Abstract
DA - 2000 Sep
IS - 3
J2 - Southeast Asian J. Trop. Med. Public Health
LA - eng
N2 - <p>A new finding is reported of multiple mutations in the rpoB gene of 9 Mycobacterium leprae strains from leprosy patients in Thailand, who did not respond to therapy even when rifampicin, the main drug in multi-drug therapy was used. By means of sequence analysis of 9 Thai M. leprae strains, various mutations in 289 bps of the rpoB gene revealed forms of mutation never before described, such as multiple mutations (ie, mutation at two, three, six, seven, eight and nine positions in the rpoB gene), most of which were point-mutation substitutions (a few of which were silent), and some insertions. This investigation demonstrates that mutation in the rpoB gene of M. leprae strains from Thailand involves more variety than previously reported for rpoB mutation patterns in rifampicin resistance M. leprae strains.</p>
PY - 2000
SP - 493
EP - 7
T2 - The Southeast Asian journal of tropical medicine and public health
TI - Multiple mutations in the rpoB gene of Mycobacterium leprae strains from leprosy patients in Thailand.
UR - http://www.tm.mahidol.ac.th/seameo/2000_31_3/15-2578.pdf
VL - 31
SN - 0125-1562
ER -