TY - JOUR
KW - Antibodies, Bacterial
KW - Enzyme-Linked Immunosorbent Assay
KW - Humans
KW - leprosy
KW - Mycobacterium tuberculosis
KW - Phosphatidylinositols
KW - Sputum
KW - Tuberculin
KW - Tuberculosis
AU - Mehta P K
AU - Khuller G K
AB - <p>The serological response to mannophosphoinositides of Mycobacterium tuberculosis H37Rv and to tuberculin-purified protein derivative (PPD) was examined by enzyme-linked immunosorbent assay (ELISA) in patients suffering from tuberculosis and related diseases. In sputum positive cases 94% samples were found to be positive to mannoside antigens and 77% to PPD, while in sputum negative cases, 71% of samples gave a positive reaction to mannosides and 54% to PPD. The high specificity of mannoside ELISA was demonstrated to be 97% in healthy individuals and 100% in patients suffering from other respiratory diseases, whereas PPD ELISA was 84% and 82% in healthy and infected patients respectively. Thus, ELISA is more specific and sensitive for mannosides than for PPD for the diagnosis of tuberculosis. However, antibodies to mannosides and PPD were detected in lepromatous as well as tuberculoid leprosy patients.</p>
BT - Medical microbiology and immunology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2845238?dopt=Abstract
DA - 1988
DO - 10.1007/bf00189413
IS - 5
J2 - Med. Microbiol. Immunol.
LA - eng
N2 - <p>The serological response to mannophosphoinositides of Mycobacterium tuberculosis H37Rv and to tuberculin-purified protein derivative (PPD) was examined by enzyme-linked immunosorbent assay (ELISA) in patients suffering from tuberculosis and related diseases. In sputum positive cases 94% samples were found to be positive to mannoside antigens and 77% to PPD, while in sputum negative cases, 71% of samples gave a positive reaction to mannosides and 54% to PPD. The high specificity of mannoside ELISA was demonstrated to be 97% in healthy individuals and 100% in patients suffering from other respiratory diseases, whereas PPD ELISA was 84% and 82% in healthy and infected patients respectively. Thus, ELISA is more specific and sensitive for mannosides than for PPD for the diagnosis of tuberculosis. However, antibodies to mannosides and PPD were detected in lepromatous as well as tuberculoid leprosy patients.</p>
PY - 1988
SP - 285
EP - 92
T2 - Medical microbiology and immunology
TI - Serodiagnostic potentialities of enzyme-linked immunosorbent assay (ELISA) using mannophosphoinositides of Mycobacterium tuberculosis H37Rv.
VL - 177
SN - 0300-8584
ER -