TY - JOUR
KW - Blood Specimen Collection
KW - Disaccharides
KW - Enzyme-Linked Immunosorbent Assay
KW - False Negative Reactions
KW - Glycolipids
KW - Humans
KW - leprosy
KW - Serologic Tests
KW - Serum Albumin, Bovine
AU - Dhandayuthapani S
AU - Anandan D
AU - Vasanthi B
AU - Bhatia V N
AB - <p>Blood samples were collected from 59 leprosy patients and 35 normal healthy subjects by veni-puncture and finger prick methods to obtain serum samples and blood spots on filter paper respectively. The serum samples at 1:300 dilutions and the eluates of dried blood spots at 1:40, 1:80, 1:160 and 1:320 dilutions were applied in ELISA to measure the antibody levels (IgM) against synthetic ND-O-BSA antigen. The antibody levels were found to be high in the multibacillary leprosy patients than the pauci-bacillary patients irrespective of whether serum samples or eluates were used. The OD values obtained at 1:160 dilution of the eluates were equivalent to that of values obtained at 1:300 dilution of the serum samples. The positivities differ in different dilution of the eluates, showing the highest in the 1:40 dilution and the lowest in the 1:320 dilution.</p>
BT - The Indian journal of medical research
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2777344?dopt=Abstract
DA - 1989 May
J2 - Indian J. Med. Res.
LA - eng
N2 - <p>Blood samples were collected from 59 leprosy patients and 35 normal healthy subjects by veni-puncture and finger prick methods to obtain serum samples and blood spots on filter paper respectively. The serum samples at 1:300 dilutions and the eluates of dried blood spots at 1:40, 1:80, 1:160 and 1:320 dilutions were applied in ELISA to measure the antibody levels (IgM) against synthetic ND-O-BSA antigen. The antibody levels were found to be high in the multibacillary leprosy patients than the pauci-bacillary patients irrespective of whether serum samples or eluates were used. The OD values obtained at 1:160 dilution of the eluates were equivalent to that of values obtained at 1:300 dilution of the serum samples. The positivities differ in different dilution of the eluates, showing the highest in the 1:40 dilution and the lowest in the 1:320 dilution.</p>
PY - 1989
SP - 150
EP - 7
T2 - The Indian journal of medical research
TI - Use of eluates of filter paper blood spots in ELISA for the serodiagnosis of leprosy.
VL - 89
SN - 0971-5916
ER -