01790nas a2200313   4500000000100000008004100001260001300042653002700055653001200082653003700094653001100131653002300142653001200165653003200177653002500209653001500234100001500249700001300264700002000277700001500297700001400312700001800326245009800344300001100442490000700453050003200460520097000492022001401462       1989                            d  c1989 Jul10aAdenosine Triphosphate10aAnimals10aDose-Response Relationship, Drug10aHumans10aLeprostatic Agents10aleprosy10aMicrobial Sensitivity Tests10aMycobacterium leprae10aPhotometry1 aKatoch V M1 aKatoch K1 aShivannavar C T1 aSharma V D1 aPatil M A1 aBharadwaj V P00aApplication of ATP assay for in vitro drug screening testing against human derived M. leprae.  a333-440 v61  aInfolep Library - available3 a<p>In this study, the ATP content of M. leprae exposed to various antimicrobial agents has been measured to evaluate its usefulness in drug sensitivity screening. Purified M. leprae suspensions from human biopsies have been incubated at 30 degrees C in a modified Dubos medium in the presence of different concentrations of various drugs viz., Rifampicin, Ethionamide, Ethambutol, Cycloserine, Dapsone, Clofazimine, Erythromycin and Tetracycline. ATP levels were estimated at 0, 7 days, 14 days of incubation by the procedures modified and standardised at this laboratory. ATP decay was accelerated by ethionamide, rifampicin, clofazimine, dapsone, erythromycin and to a lesser extent by cycloserine, whereas ethambutol and tetracycline did not have any significant effect. The rate of decay depended on the concentrations of these drugs. ATP assay promises to be a useful system for in vitro drug sensitivity screening against M. leprae isolated from patients.</p>  a0254-9395