01732nas a2200313   4500000000100000008004100001260001300042653002400055653001600079653001400095653001100109653001200120653002600132653002500158653003100183653001800214100001200232700001500244700001400259700001600273700001400289700001500303700001700318245009700335300001200432490000700444520095300451022001401404       1989                            d  c1989 Aug10aAntigens, Bacterial10aBCG Vaccine10aCell Line10aHumans10aleprosy10aLymphocyte Activation10aMycobacterium leprae10aReproducibility of Results10aT-Lymphocytes1 aLee S P1 aStoker N G1 aGrant K A1 aHandzel Z T1 aHussain R1 aMcAdam K P1 aDockrell H M00aCellular immune responses of leprosy contacts to fractionated Mycobacterium leprae antigens.  a2475-800 v573 a<p>Antigens of armadillo-derived Mycobacterium leprae sonic extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane, and the unstained blot was converted into 20 fractions of antigen-bearing particles. These were tested in cellular proliferation assays, and reproducible results were obtained between batches of fractions. Peripheral blood mononuclear cells from healthy contacts of leprosy patients (presumed to have protective immunity) were tested with the fractions to investigate which antigens they recognized. A small group of tuberculoid leprosy patients were also tested. Both groups showed a wide range of responses. Almost every fraction stimulated proliferation with at least one donor, yet none was clearly immunodominant or inhibitory in either group. Thus, protective immunity did not appear to be associated with proliferation caused by any single fraction.</p>  a0019-9567