02245nas a2200421   4500000000100000008004100001260001300042653001500055653001000070653000900080653002500089653002600114653002700140653002400167653001000191653001100201653003500212653001100247653002800258653002500286653001200311653000900323653001600332653002500348100001800373700001800391700001400409700001300423700001600436700001300452700001500465245011200480856004100592300001000633490000700643520115900650022001401809       1989                            d  c1989 Sep10aAdolescent10aAdult10aAged10aAlkaline Phosphatase10aAntibodies, Bacterial10aAntibodies, Monoclonal10aAntigens, Bacterial10aChild10aFemale10aFluorescent Antibody Technique10aHumans10aImmunoenzyme Techniques10aImmunohistochemistry10aleprosy10aMale10aMiddle Aged10aMycobacterium leprae1 aKhanolkar S R1 aMackenzie C D1 aLucas S B1 aHussen A1 aGirdhar B K1 aKatoch K1 aMcAdam K P00aIdentification of Mycobacterium leprae antigens in tissues of leprosy patients using monoclonal antibodies.  uhttp://ila.ilsl.br/pdfs/v57n3a09.pdf  a652-80 v573 a<p>Five monoclonal antibodies (MAbs) directed against antigens of Mycobacterium leprae were tested for their ability to bind to components of tissue sections prepared from biopsies taken from patients with various forms of leprosy. Immunoperoxidase was the most successful marker system used, although immunofluorescence and alkaline phosphatase were also successful in certain cases. Positivity was high with all five antibodies successfully staining those sections containing a bacterial index of 3+ or more; sections with 0 bacterial counts also had areas staining positively with two of the MAbs. The positive staining in the tissues was confined to areas infiltrated by inflammatory cells; however it was not identifiable as being associated with individual bacteria. These findings suggest that immunostaining with specific monoclonal antibodies can help to identify leprosy in diagnostic samples in which acid-fast bacilli are not identifiable by standard histochemical means. Immunohistochemical techniques are likely to be valuable in studies of the distribution of M. leprae antigens and their association with individual tissue elements.</p>
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