01694nas a2200289   4500000000100000008004100001260000900042653001400051653001100065653002500076653002500101653001600126653003900142653001900181653001700200653001800217653001800235100001300253700001800266700001700284700001600301245008900317300000900406490000600415520096900421022001401390       1989                            d  c198910aGranuloma10aHumans10aLeprosy, lepromatous10aLeprosy, Tuberculoid10aMacrophages10aPeripheral Nervous System Diseases10aPeroneal Nerve10aRadial Nerve10aSpinal Nerves10aT-Lymphocytes1 aKehrer D1 aNarayanan R B1 aMalaviya G N1 aGirdhar B K00aIsolation and characterization of cells in granulomas of nerves of leprosy patients.  a7-110 v73 a<p>Single cell suspension from the granulomas in nerves of leprosy patients were prepared for an in vitro study of the properties of infiltrating cells. Nerve biopsies from 17 patients with tuberculoid (n = 9) and lepromatous (n = 8) leprosy cases were analysed. The granulomas were found to contain lymphocytes and macrophages. Lymphocytes were the predominant infiltrating cells in the tuberculoid nerves. In contrast, lepromatous nerves contained very few of these cells. The majority of lymphocytes in tuberculoid granulomas were activated T cells as they formed rosettes with sheep erythrocytes, exhibited esterase dots in the cytoplasm and expressed HLA-DR antigens. A small proportion of the lymphocytes also formed rosettes with EAC. Most macrophages from both the granulomas were mature macrophages as they were esterase positive, did not exhibit peroxidase activity and expressed HLA-DR antigens. The macrophages did not possess C3 surface receptors.</p>  a0001-5938