01998nas a2200325   4500000000100000008004100001260000900042653001500051653001000066653000900076653002900085653001000114653002100124653004000145653001100185653001100196653001200207653000900219653001600228653001400244100001800258700001400276700001400290700001500304245010300319300001100422490000700433520121800440022001401658       1989                            d  c198910aAdolescent10aAdult10aAged10aAntigen-Antibody Complex10aChild10aChild, Preschool10aElectrophoresis, Polyacrylamide Gel10aFemale10aHumans10aleprosy10aMale10aMiddle Aged10aNeoplasms1 aSegal-Eiras A1 aCroce M V1 aRamos G S1 aCórsico B00a[Isolation and characterization of immune complexes associated with malignant tumors and leprosy].  a125-300 v493 a<p>We are dedicated to the study of circulating immune complexes (CIC) associated with different diseases: malignant tumors, leprosy and rheumatoid arthritis. Immune complexes were evaluated by various methods: 125I-Clq binding assay, 125I-IgG binding test, 125I-bovine conglutinin binding assay and polyethylene glycol precipitation test (3.5% and 2.5%). Techniques for the isolation and splitting of CIC in their components were performed in sera from patients with tumors and with leprosy. These methods consisted in the combination of CIC with protein A followed by elution with different buffers. CIC splitting techniques were first applied on immune complexes formed in vitro (BSA-aBSA, OVA-aOVA). The analysis of CIC fractions was done by SDS-PAGE, immunoelectrophoresis and immunoblotting techniques. Results were as follows: CIC levels correlated with active stages of disease, decreasing during remission so that CIC detection can be useful to evaluate response to treatment. The isolation and splitting of immune complexes into their components resulted in the obtention of immunologically active fractions, especially in sera from patients with gastrointestinal and breast cancer and with leprosy.</p>  a0025-7680