01512nas a2200301   4500000000100000008004100001260001300042653001200055653001500067653002300082653002000105653002100125653001100146653001800157653002500175653003100200653001300231653004600244653002400290653002400314100002200338700001400360245007800374300001200452490000800464520072400472022001401196       1989                            d  c1989 Sep10aAnimals10aArmadillos10aCloning, Molecular10aGene Conversion10aGenes, Bacterial10aHumans10aMycobacterium10aMycobacterium leprae10aNucleic Acid Hybridization10aPlasmids10aPolymorphism, Restriction Fragment Length10aRestriction Mapping10aSpecies Specificity1 aClark-Curtiss J E1 aWalsh G P00aConservation of genomic sequences among isolates of Mycobacterium leprae.  a4844-510 v1713 a<p>Restriction fragment length polymorphism analysis has been used to assess relatedness among the genomes of four isolates of Mycobacterium leprae, the causative agent of leprosy. The M. leprae isolates were from human patients from India, a Mangabey monkey from West Africa, and an armadillo from Louisiana. A total of 16 probes were used; these were insert fragments of M. leprae DNA from plasmid recombinant libraries, 5 of which had genes with identifiable functions and 11 of which were randomly chosen recombinant molecules. In spite of the widely diverse origins of the isolates, restriction fragment length polymorphism analysis demonstrated that less than 0.3% of the nucleotides differ among the genomes.</p>  a0021-9193