02122nas a2200205   4500000000100000008004100001260001200042653002200054653001400076653001200090653001500102100001000117700001000127245017200137856015300309300001100462490000800473520142100481022001401902       2023                            d  c04/202310aCD11b(+)Gr-1(low)10aGranuloma10aM-MDSCs10atratamento1 aJin S1 aLee S00aCD11bGr-1 cells that accumulate in M.leprae-induced granulomas of the footpad skin of nude mice have the characteristics of monocytic-myeloid-derived suppressor cells.  uhttps://www.sciencedirect.com/science/article/pii/S1472979223000434/pdfft?md5=ce02808c14239899aafda1ea76893676&pid=1-s2.0-S1472979223000434-main.pdf  a1023450 v1403 a<p>CD11bGr-1 cells that are increased in the lungs of a Mycobacterium (M) tuberculosis-infection mouse model have the characteristics of monocytic (M)-myeloid-derived suppressor cells (MDSCs) and harbor M.tuberculosis. Interestingly, a high number of M-MDSCs have also been observed in skin lesions of patients with lepromatous leprosy. We hypothesized that CD11bGr-1 cells might be involved in the pathogenesis of leprosy, as they are in tuberculosis. In the current study, we investigated the issue of whether CD11bGr-1 cells accumulate in Mycobacterium (M) leprae-induced granulomas of the footpad skin of nude mice. Our results show that CD11bGr-1 cells began to accumulate in the 7-month-old M.leprae-induced granulomas and were replaced by other leukocytes, including CD11bGr-1 over time during M.leprae infections. CD11b&nbsp;&nbsp;Gr-1 cells expressed the surface markers of M-MDSC, Ly6C and Ly6G. In addition, CD11bGr-1 cells have the nuclei of a mononuclear cell type and expressed higher levels of arginase 1 (Arg1) and inducible NO synthetase (iNOS). Furthermore, they showed a higher infection rate by M.leprae. Taken together, our results indicate that the inoculation with M.leprae induced an accumulation of CD11b&nbsp;&nbsp;Gr-1 at a relatively early stage, 7-month-old M.leprae-induced granulomas, and that CD11bGr-1 have the characteristics of M-MDSC and may act as a reservoir for M.leprae.</p>
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