01671nas a2200337   4500000000100000008004100001260001300042653001200055653001500067653001100082653002500093653001200118653002400130653002500154653002500179653001400204653003400218653003000252100001600282700001700298700001600315700001200331700001800343700001800361245013100379856004100510300001000551490000700561520075100568022001401319       1989                            d  c1989 Jun10aBlister10aCell Count10aHumans10aImmunohistochemistry10aleprosy10aLeprosy, Borderline10aLeprosy, lepromatous10aLeprosy, Tuberculoid10aMonocytes10aT-Lymphocytes, Helper-Inducer10aT-Lymphocytes, Regulatory1 aRangdaeng S1 aScollard D M1 aSuriyanon V1 aSmith T1 aThamprasert K1 aTheetranont C00aStudies of human leprosy lesions in situ using suction-induced blisters. 1. Cellular components of new, uncomplicated lesions.  uhttp://ila.ilsl.br/pdfs/v57n2a07.pdf  a492-80 v573 a<p>The cellular contents of blisters induced by suction over new, uncomplicated leprosy lesions, and in the skin of cured, control patients, have been examined with enzyme- and immuno-histochemical staining over a period of 4 days. The total cellularity of the blisters varied over a wide range, not correlated with the type of leprosy. Mononuclear cells predominated at all times studied, with nearly equal percentages of monocytes and T lymphocytes. The T-helper: suppressor ratio was significantly greater in BT than in BL and LL lesions at 48 hr. Suction blisters offer a painless, quantitative, reproducible, multiple-sampling method for obtaining cells from the cutaneous infiltrates of leprosy for phenotyping or functional analysis.</p>
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