01632nas a2200241   4500000000100000008004100001260001300042653002400055653001800079653003800097653001600135653001100151653002500162653001200187653002500199653002600224100001100250245004800261300001000309490000700319520105000326022001401376       1989                            d  c1989 Oct10aAntigens, Bacterial10aDisaccharides10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aHumans10aLatex Fixation Tests10aleprosy10aMycobacterium leprae10aSerum Albumin, Bovine1 aWu Q X00a[Study on rapid serodiagnosis for leprosy].  a391-40 v113 a<p>In this article, we have developed two latex agglutination reaction tests (LAR) with PGL-1 and ND-O-BSA as antigens in 80 cases of leprosy patients (LL = 20, BL = 20, BT = 20, TT = 20), 20 cases of tuberculosis and 25 cases of normal control. These two latex agglutination tests were compared with relevant ELISAs (ND-O-BSA- and PGL-1-ELISA), and analysed by Chi-square (chi 2) test. The results indicated that the sensitivity and specificity of the two LARs (PGL-1- and ND-O-BSA-LAR) are similar to their relevant ELISAs respectively (PGL-1-LAR Vs ND-O-BSA-LAR, chi 2 = 1.785, P greater than 0.10; PGL-1-LAR Vs PGL-1-ELISA, chi 2 = 2.0, P greater than 0.10; ND-O-BSA-LAR Vs ND-O-BSA-ELISA, chi 2 = 0.972, P greater than 0.25), and the increase of positive rate is coincident with clinical classification of leprosy. Since LARs are more simple and rapid than ELISAs, LARs may become more useful tools for immunodiagnosis of leprosy in the field. Also conditions to ensure the stability and reproducibility of LARs are discussed in detail.</p>  a1000-503X