01542nas a2200325   4500000000100000008004100001260001700042653002700059653002400086653001300110653001100123653002800134653001200162653001600174653002500190653000900215653001700224100001800241700001100259700001200270700001500282700001700297700001600314245010800330300001100438490000700449050003200456520071400488022001401202       1985                            d  c1985 Apr-Jun10aAntibodies, Monoclonal10aAntigens, Bacterial10aEpitopes10aHumans10aImmunoenzyme Techniques10aleprosy10aLymph Nodes10aMycobacterium leprae10aSkin10aTuberculosis1 aNarayanan R B1 aRamu G1 aSinha S1 aSengupta U1 aMalaviya G N1 aDesikan K V00aDemonstration of Mycobacterium leprae specific antigens in leprosy lesions using monoclonal antibodies.  a258-640 v57  aInfolep Library - available3 a<p>Cryostat sections of dermal lesions from 13 untreated patients of leprosy were studied by indirect immunoperoxidase using monoclonal antibodies (MLO4 & MLO6), defining M. leprae specific antigens. The lymphocytes and macrophages in both the tuberculoid and lepromatous granulomas showed membranous staining with the above antibodies. M. leprae organisms in the lepromatous granulomas and the cells in the section of lymph nodes of patients with tuberculosis, or sections of normal skin or psoriatic lesions did not show any staining with these antibodies. These observations suggest that M. leprae specific antigens are present and expressed on the cells infiltrating the granulomas of leprosy lesions.</p>  a0254-9395