02864nas a2200277   4500000000100000008004100001260002300042653002700065653002400092653001700116100001000133700001100143700001200154700001200166700001100178700000900189700001100198700001000209700001100219245012900230856005500359300001400414490001400428520213000442022001402572       2022                            d  bInforma UK Limited10aPharmacology (medical)10aInfectious Diseases10aPharmacology1 aShi Y1 aKong W1 aJiang H1 aZhang W1 aWang C1 aWu L1 aShen Y1 aYao Q1 aWang H00aMolecular Surveillance of Antimicrobial Resistance of Mycobacterium leprae from Leprosy Patients in Zhejiang Province, China  uhttps://www.dovepress.com/getfile.php?fileID=82427  a4029-40360 vVolume 153 a<p><strong>Purpose:</strong>&nbsp;Reports on antimicrobial resistance (AMR) of Mycobacterium&nbsp;<em>leprae</em>&nbsp;(<em>M. leprae</em>) in Zhejiang Province are limited. Thus, this study aimed to investigate the drug resistance of new leprosy cases within several years and analyse the emergence of AMR mutations from Zhejiang Province.<br />
<strong>Methods:</strong>&nbsp;This study enrolled 34 leprosy cases in Zhejiang Province, China, from 2018 to 2021. Gene mutation of WHO-recommended DRDRs (<em>fol</em>P1,&nbsp;<em>rpo</em>B and&nbsp;<em>gyr</em>A) and genes of compensatory AMR-associated DRDRs, including nth,&nbsp;<em>rpo</em>A,&nbsp;<em>rpo</em>C,&nbsp;<em>gyr</em>B and 23S rRNA, were detected by amplification. Clinical data analysis was performed to investigate the epidemiological association of leprosy.<br />
<strong>Results:</strong>&nbsp;Of the 34 samples, 2 (5.9%) strains showed drug resistance, which were mutated to dapsone and ofloxacin, separately. Two single mutations in&nbsp;<em>gyr</em>B were detected in different strains (5.9%), whereas one of the&nbsp;<em>rpo</em>C mutation was also detected in one strain each (2.9%), which were proved to be polymorphs. No correlation of drug resistance proportion was identified in male vs female, nerve vs no nerve involvement, deformity vs no deformity and reaction vs non-reaction cases.<br />
<strong>Conclusion:</strong>&nbsp;Results showed well control of leprosy patients in Zhejiang Province. Gene mutations of WHO-recommended DRDRs&nbsp;<em>fol</em>P1 and&nbsp;<em>gyr</em>A confirmed the resistance to dapsone and ofloxacin. Compensatory AMR-associated mutations confirmed to be polymorphs still require further study to determine their phenotypic outcomes in&nbsp;<em>M. leprae</em>. The results demonstrated that drug-resistant strains are not epidemic in this area. Given the few cases of leprosy, analysing the AMR of&nbsp;<em>M. leprae</em>&nbsp;in Zhejiang Province more comprehensively is difficult. However, regular MDT treatment and population management in the early stage may contribute to the low prevalence of leprosy.</p>
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