01816nas a2200265   4500000000100000008004100001260001200042653001900054653002500073653001500098653001500113653003200128100001300160700001600173700001200189700001400201700001400215700001500229700001100244700001700255245014600272300001100418520110700429022001401536       2022                            d  c05/202210aCanine leprosy10aCutaneous granulomas10aDermatitis10aHsp65 gene10aNontuberculous Mycobacteria1 aBiezus G1 ade Cristo T1 aIkuta C1 aCarniel F1 aVolpato J1 aTeixeira M1 aNeto J1 aCasagrande R00aCanine leproid granuloma (CLG) caused by mycobacterial species closely related to members of Mycobacterium simiae complex in a dog in Brazil.  a1006723 a<p>This report describes the clinical features and molecular diagnosis of a case of canine leproid granuloma (CLG) caused by mycobacterial strains of the Mycobacterium simiae complex in Brazil. A 12-year-old non-neutered male Labrador Retriever dog was presented with a two-week history of progressive painless cutaneous lesions. Ulcerated nodules with hematic crusts were observed on the dorsal surface of the right and left pinna and on the metacarpal, metatarsal, and digits. Complete blood count, serum biochemistry, aspiration cytology of cutaneous lesions, biopsy for histopathological evaluation, culture for aerobic and anaerobic bacteria, polymerase chain reaction (PCR) and DNA sequencing to identify mycobacterial species were performed. According to the clinical and histopathological findings, a diagnosis of CLG was established. Despite the negative result of the bacterial culture, mycobacterial identification was made by sequencing the hsp65 gene. Our findings highlight that mycobacterial species closely related to members of the M. simiae clade can be causative agents of CLG.</p>
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